Trihybrid cross calculator

Three independently assorting genes, 64 combinations — worked out instantly. Pick each parent’s genotype for traits A, B and C.

♀ Mother
♂ Father
Quick set:
Name your traits (optional)
A/a
B/b
C/c
Phenotype ratio
Genotype ratio
Cross

Phenotypes

Gametes

Punnett square

Columns are the mother's (♀) gametes, rows the father's (♂). Cells are coloured by phenotype.

Genotypes

Using the product rule to check your answer

Because the three genes assort independently, any offspring probability is the product of three single-gene probabilities. For AaBbCc × AaBbCc:

Two genes are easier to visualise — try the dihybrid cross calculator.

FAQ

How many boxes are in a trihybrid Punnett square?

64. Each fully heterozygous parent (AaBbCc) makes 2³ = 8 different gametes, and 8 × 8 = 64 combinations.

What is the trihybrid phenotype ratio?

For AaBbCc × AaBbCc it is 27 : 9 : 9 : 9 : 3 : 3 : 3 : 1 — eight phenotype classes. That is (3:1)³ expanded. The calculator lists them in a fixed trait order, so the 9s and 3s may appear interleaved.

Is there a shortcut instead of drawing 64 boxes?

Yes — the product rule. Treat each gene as its own monohybrid cross and multiply probabilities. For example, P(aabbcc) = ¼ × ¼ × ¼ = 1/64.